Please confirm topic selection

Are you sure you want to trigger topic in your Anconeus AI algorithm?

Please confirm action

You are done for today with this topic.

Would you like to start learning session with this topic items scheduled for future?

Updated: Aug 19 2022

Special Culture Requirements

  • Overview
    • Haemophilus influenzae
      • chocolate agar
        • factor V (NAD+)
        • factor X (hematin, a precursor of hemin)
    • Neisseria gonorrhoeae
      • Thayer-Martin (VPN) media
      • selects for N. gonorrhoeae growth only
        • vancomycin
          • inhibits gram-positive organisms
        • polymyxin
          • inhibits gram-negative organisms
        • nystatin
          • inhibits fungi
    • Mycoplasma
      • cholesterol
    • Salt
      • Staphylococcus aureus can grow in high levels of salt
      • Vibrio spp. requires NaCl to grow and grows in 6.5% NaCl
      • Enterococci (group D streptococci) grows in 6.5% NaCl
        • differentiates from non-enterococcal group D strepococci (S. bovis)
    • Bile
      • group D streptococci can grow in bile (both Streptococcus bovis and Enterococci)
      • Klebsiella can grow in bile
    • Mycobacterium tuberculosis
      • Löwenstein-Jensen agar
    • Mycoplasma pneumoniae
      • Eaton's agar
      • Mycoplasma must be given a long time to grow
    • Bordetella pertussis
      • collect culture with calcium alginate swab
      • Regan-Lowe medium
      • Bordet-Gengou (potato) agar
        • "BORDETella"
    • Corynebacterium diphtheriae
      • Cysteine-tellurite agar
        • forms metachromatic granules detected with methylene blue stain
      • Löffler's media
    • Fungi
      • Sabouraud's agar
        • especially used for dermatophytes
      • fungi causing systemic infections must be given a long time to grow!
        • e.g. Blastomyces, Histoplasma, Coccidioides
    • Legionella
      • charcoal yeast extract agar with cysteine and iron
    • Cysteine required
      • Francisella
      • Brucella
      • Legionella
      • Pasteurella
    • Lactose-fermenting enterics
      • MacConkey's agar
        • pink colonies
        • due to acid production by fermentation
        • contains bile to inhibit growth of unwanted organisms
      • EMB agar (eosin-methylene blue)
        • also used to differentiate lactose-fermenting bacteria
        • Escherichia coli appears with metallic green sheen
1 of 0
1 of 3
Private Note

Attach Treatment Poll
Treatment poll is required to gain more useful feedback from members.
Please enter Question Text
Please enter at least 2 unique options
Please enter at least 2 unique options
Please enter at least 2 unique options