Overview Function replicate cell genome in a manner that is highly accurate Process DNA melted to expose single strand to expose origin of replication single stranded binding proteins (SSBs) bind and stabilize melted DNA RNA primer added in 5' → 3' direction by primase DNA polymerase adds adds nucleotides in a 5' → 3' login to view 5 more bullets DNA gyrase (topoisomerase II) breaks the DNA to prevent coiling RNA primer removed login to view 2 more bullets DNA ligase seals the nick between fragments Differences between prokaryotes eukaryotes prokaryotes login to view 1 more bullet eukaryotes login to view 1 more bullet Clinical importance antibiotics login to view 3 more bullets cancer chemotherapy login to view 1 more bullet