Overview Function de novo glucose synthesis effectively glycolysis in reverse can maintain blood glucose when glycogen stores are exhausted must supply brain and RBCs which utilize glucose for energy login to view 2 more bullets potential substrates all amino acids login to view 3 more bullets lactate login to view 1 more bullet glycerol-3-phosphate login to view 1 more bullet propionyl-CoA login to view 1 more bullet Pathway location hepatocytes (primary) kidney enterocytes NOT muscle login to view 2 more bullets enzymes involves both mitochondrial and cytosolic enzymes several steps of glycolysis are reversible the non-reversible steps must be bypassed with special gluconeogenic enzymes login to view 22 more bullets other enzymes login to view 3 more bullets regulation stimulation login to view 3 more bullets inhibition login to view 2 more bullets