Ribosome Structure eukaryotes login to view 6 more bullets prokaryotes login to view 1 more bullet Catalytic sites A (acceptor) site login to view 1 more bullet P (peptidyl) site login to view 1 more bullet E (exit) site login to view 1 more bullet Forms polysomes login to view 7 more bullets Steps Initiation initiation complex generated from login to view 10 more bullets once assembled, recruits the large ribosomal subunit login to view 3 more bullets Elongation aminoacyl-tRNA binds to A site login to view 1 more bullet peptidyl-bond formation login to view 2 more bullets translocation login to view 7 more bullets cycle is repeated for each amino acid addition to the polypeptide Wobble phenomenon login to view 2 more bullets Termination stop codon reached in mRNA release factor binds mRNA peptide bond is hydrolyzed and completed protein is released ribosomal complex dissociates Overall energy requirement 4 high energy bonds login to view 2 more bullets Pharmacologic Inhibition of Prokaryotic Protein Synthesis Bind 30S aminoglycosides login to view 2 more bullets Bind 50S chloramphenicol login to view 1 more bullet macrolides login to view 1 more bullet clindamycin login to view 1 more bullet