Overview Function determining sequence for a desired DNA sample Process (Sanger/direct method) DNA sample put in 4 different reaction mixtures login to view 1 more bullet dideoxynucleotides stop DNA polymerization at each base login to view 1 more bullet solutions are separated by gel electrophoresis sequence can be deduced by looking at each electrophoresis row and reading which ddNTP was added to that tube column Clinical use most commonly used when no specific set of mutations is a common cause of disease