Please confirm topic selection

Are you sure you want to trigger topic in your Anconeus AI algorithm?

Please confirm action

You are done for today with this topic.

Would you like to start learning session with this topic items scheduled for future?

Updated: May 5 2017

DNA Sequencing

  • Overview
    • Function
      • determining sequence for a desired DNA sample
    • Process (Sanger/direct method)
      • DNA sample put in 4 different reaction mixtures
        • each contains all 4 deoxynucleotides, 1 different dideoxyneucleotide (ddNTP), and a DNA polymerase
      • dideoxynucleotides stop DNA polymerization at each base
        • generates DNA chains of various lengths that encompass the entire original sequence
      • solutions are separated by gel electrophoresis
      • sequence can be deduced by looking at each electrophoresis row and reading which ddNTP was added to that tube column
    • Clinical use
      • most commonly used when no specific set of mutations is a common cause of disease
1 of 0
Private Note

Attach Treatment Poll
Treatment poll is required to gain more useful feedback from members.
Please enter Question Text
Please enter at least 2 unique options
Please enter at least 2 unique options
Please enter at least 2 unique options