Prokaryotic Overview 1 type of RNA polymerase (RNAp) Structure α2ββ' core subunits Initiation σ (sigma) = initiation factor binds RNAp to DNA Termination ρ (rho) = termination factor Clinical importance inhibited by rifampin login to view 1 more bullet actinomycin D login to view 1 more bullet Eukaryotic Overview 3 types of RNA polymerase RNAp 1 synthesizes rRNA in nucleolus remember: rRNA is most abundant RNA; so it is #1 RNAp 2 synthesizes mRNA, snRNA in nucleus RNAp 3 synthesizes tRNA in nucleus remember: tee = three makes eukaryotic 5s rRNA Structure similar in structure to prokaryotes Initiation TFIID = initiation factor binds RNAp to DNA Termination translation continues far past gene and RNAp eventually falls off Clinical importance inhibited by α-amanitin login to view 3 more bullets actinomycin D login to view 1 more bullet