Blotting Procedures Function probe for specific substance in solution Southern blot - used to analyze DNA login to view 2 more bullets Northern blot - used to analyze RNA login to view 1 more bullet Western blot (immunoblot) - used to analyze protein Process run a gel electrophoresis to separate the components of the solution bands are transferred ("blotted") to a filter/membrane radiolabeled or fluorescently labeled probe is incubated with the membrane Southern blot - 32P-DNA Northern blot - 32P-DNA Western blot - enzyme-linked or 131I antibody specific probe binds with high specificity to DNA/RNA segment or protein of interest membrane is visualized under conditions to illuminate the probe Clinical use restriction length polymorphism (RFLP) can be used for genetic testing based on the principle that individuals have unique/heritable variations in RFLPs login to view 2 more bullets Southern blot used to visualize results ex.) testing for sickle-cell anemia login to view 6 more bullets gene expression profiling measure whether a particular region of DNA is being expressed Northern blot used to visualize results ex.) testing for fragile X syndrome login to view 2 more bullets