Overview Process initiation RNAp binds the template DNA strand at the promoter site with the help of transcription factors elongation RNAp synthesizes the mRNA strand in 5' → 3' direction reads template strand in 3' → 5' direction eukaryotic RNAps transcribe introns as well as exons RNAp inhibited by alpha-amanitin (ingestion of the amanita phalloides mushroom) termination prokaryotes two mechanisms rho-dependent rho protein binds the mRNA causes RNAp to fall off rho-independent mRNA has a GC-rich segment that naturally forms a hairpin causes RNAp to fall off eukaryotes transcription continues past the gene and eventually falls off Mono/polycistronic monocistronic = mRNA codes for 1 protein eukaryotes only have monocistronic mRNA polycistronic = mRNA codes for >1 protein e.g. bacterial lac operon
QUESTIONS 1 of 3 1 2 3 Previous Next Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK (M1.BC.15.74) E. coli has the ability to regulate its enzymes to break down various sources of energy when available. It prevents waste by the use of the lac operon, which encodes a polycistronic transcript. At a low concentration of glucose and absence of lactose, which of the following occurs? QID: 106639 Type & Select Correct Answer 1 Increased allolactose levels bind to the repressor 6% (20/354) 2 Increased cAMP levels result in binding to the catabolite activator protein 47% (166/354) 3 Decreased cAMP levels result in poor binding to the catabolite activator protein 29% (104/354) 4 Trascription of the lac Z, Y, and A genes increase 4% (13/354) 5 Repressor releases from lac operator 11% (39/354) M 1 Question Complexity D Question Importance Select Answer to see Preferred Response SUBMIT RESPONSE 2 Review Tested Concept Review Full Topic Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK
All Videos (2) Login to View Community Videos Login to View Community Videos Transcription 2 Keshav Mudgal Biochemistry - Transcription D 1/14/2016 62 views 5.0 (1) Login to View Community Videos Login to View Community Videos Transcription 1 Keshav Mudgal Biochemistry - Transcription D 1/14/2016 99 views 4.0 (1) Biochemistry | Transcription Biochemistry - Transcription Listen Now 9:32 min 11/27/2022 53 plays 0.0 (0)