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Updated: Aug 21 2018

Apolipoproteins

  • Overview
  • Snapshot
    • A 50-year-old woman presents to her primary care physician for a regular checkup. She states that for the past four months she has not experienced menses. Her primary care physician suspects that she has gone through menopause. On routine lab work, her LDL has increased as compared to one year ago, and her HDL has decreased drastically.
  • Introduction
    • Mechanisms for fatty acid and cholesterol transport
    • Different types that vary by
      • density
        • most dense
          • HDL (high density)
          • LDL (low density)
          • IDL (intermediate density)
          • VLDL (very low density)
          • chylomicrons
        • least dense
      • types of apolipoprotein
  • HDL
    • Function
      • transfer cholesterol
        • tissues → liver
      • good cholesterol
    • Structure
      • apoA-1
        • activates lecithin cholesterol acyltransferase (LCAT)
      • apoE/apoC-II
        • apolipoproteins donated to chylomicrons and VLDL
  • LDL
    • Function
      • transfer cholesterol
        • liver → tissues
          • most recycles back to be absorbed by the liver
      • bad cholesterol
    • Structure
      • apoB-100
        • mediates endocytosis of LDL by binding to apoB-100 (LDL) receptor on liver and tissues
  • IDL
    • Function
      • VLDL → IDL + TGs
      • picks up cholesterol esters from HDL
        • catalyzed by cholesterol ester transfer protein (CETP)
        • IDL + cholesterol from HDL → LDL
    • Structure
      • apoE
        • recieves from HDL
        • mediates uptake by the liver
        • remember: apoE empties to liver
      • apoB-100
        • mediates endocytosis of LDL by binding to apoB-100 (LDL) receptor on liver and tissues
  • VLDL
    • Function
      • transports TGs
        • liver → tissues
    • Structure
      • apoB-100
        • form packaged and secreted from liver
      • apoC-II
        • activates lipoprotein lipase
          • catalyzes hydrolysis of triglycerides into individual FAs for absorbtion
          • ↑ regulated by insulin
      • apoE
        • mediates uptake by the liver
  • Chylomicrons
    • Function
      • transport cholesterol, TGs, FAs, and fat soluble vitamins
        • intestine → tissues
      • released from intestinal lumen cells into lymphatics
    • Structure
      • apo-48
        • form packaged and secreted by intestine
      • apoC-II
        • activates lipoprotein lipase
        • added from HDL
      • apoE
        • mediates uptake by the liver
        • added from HDL
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