Overview Function couple energy stored in electron acceptors (NADH, FADH2) to ATP synthesis process called oxidative phosphorylation login to view 6 more bullets Pathway located in inner mitochondrial membrane series of carrier enzymes NADH and FADH2 create a proton gradient across the inner membrane pass electrons in a stepwise fashion oxygen is the final electron acceptor flow of proton back down concentration gradient drives F0F1 ATP synthase complex login to view 1 more bullet Clinical importance electron transport inhibitors disrupt membrane bound carrier enzymes login to view 5 more bullets CO login to view 13 more bullets CN login to view 16 more bullets note: victims of house fires may have both CO and CN poisoning ATPase inhibitors directly inhibit mitochondrial ATP synthase login to view 3 more bullets e.g. oligomycin uncoupling agents "uncouples" ATP production from the proton gradient ↑ permeability of membrane login to view 12 more bullets Compound Site of inhibition CO (carbon monoxide) Cytochrome c CN (cyanide) Cytochrome c Antimycin Cytochrome b/c1 Doxorubicin CoQ Rotenone (pesticide) NADH dehydrogenase