Overview Only in eukaryotes pre-processed transcript = hnRNA Process capping 5' end 7-methylguanosine cap occurs before transcription has finished functions to help in ribosomal binding also protects against degradation 3' end polyadenylated (poly-A) tail added by poly-A-polymerase functions to protect from degradation some viruses can steal host cell caps so that the viral mRNA gets translated splicing process of intron removal mediated by the spliceosome composed of small nuclear ribonucleoproteins (snRNPs) lariat is formed adjacent exons are attached alternative splicing one hnRNA can be spliced differently to produce different protein products this requires conserved sequences including GU at the beginning of the intron AG at the end of the intron mutations that create new splice sites (AG) in the middle of the intron can lead to abberant splicing that creates new nonfunctional proteins longer proteins than the wildtype variant Location occurs in nucleus after transcription only processed RNA is transported out of the nucleus
QUESTIONS 1 of 7 1 2 3 4 5 6 7 Previous Next Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK (M1.BC.15.72) Expression of an mRNA encoding for a soluble form of the Fas protein prevents a cell from undergoing programmed cell death. However, after inclusion of a certain exon, this same Fas pre-mRNA eventually leads to the translation of a protein that is membrane bound, subsequently promoting the cell to undergo apoptosis. Which of the following best explains this finding? QID: 106503 Type & Select Correct Answer 1 Base excision repair 2% (9/371) 2 Histone deacetylation 3% (12/371) 3 DNA missense mutation 2% (9/371) 4 Post-translational modifications 15% (55/371) 5 Alternative splicing 76% (283/371) M 1 Question Complexity D Question Importance Select Answer to see Preferred Response SUBMIT RESPONSE 5 Review Tested Concept Review Full Topic (M1.BC.15.1) Hepatocyte nuclear factor 4 alpha (HNF4a) is a transcription factor that is found in the liver, pancreas, kidney, and intestines. The gene is composed of 11 exons and depending on the tissue there are different isoforms of the protein being expressed. Which of the following is responsible for producing the different isoforms of HNF4a? QID: 106969 Type & Select Correct Answer 1 Addition of 7-methylguanosine cap 0% (0/117) 2 Addition of polyadenylated (poly-A) tail 1% (1/117) 3 Alternative splicing 95% (111/117) 4 Histone acetylation 3% (3/117) 5 DNA methylation 2% (2/117) M 1 Question Complexity D Question Importance Select Answer to see Preferred Response SUBMIT RESPONSE 3 Review Tested Concept Review Full Topic (M1.BC.14.24) A 30-year-old African American woman develops a facial rash in a "butterfly" pattern over her face and complains of feeling tired and achy in her joints. In the course of a full rheumatologic workup you note that she has anti-snRNP antibodies. Which of the following do snRNPs affect? QID: 100089 Type & Select Correct Answer 1 Addition of the 5' 7-methylguanosine cap of mRNA 3% (4/118) 2 Polyadenylation of the 3' end of mRNA 2% (2/118) 3 Protection of mRNA from degradation 10% (12/118) 4 Intron removal from the mRNA 75% (88/118) 5 Transcription of mRNA 5% (6/118) M 1 Question Complexity C Question Importance Select Answer to see Preferred Response SUBMIT RESPONSE 4 Review Tested Concept Review Full Topic Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK
All Videos (2) Login to View Community Videos Login to View Community Videos Introns and Exons Keshav Mudgal Biochemistry - RNA Processing D 1/14/2016 62 views 5.0 (1) Login to View Community Videos Login to View Community Videos RNA processing Biochemistry - RNA Processing D 2/14/2015 133 views 5.0 (4) Biochemistry | RNA Processing Biochemistry - RNA Processing Listen Now 11:49 min 8/15/2021 59 plays 3.0 (1)