Updated: 5/5/2017

DNA Sequencing

0%
Topic
Review Topic
0
0
0%
0%
Videos
2 2
Overview
  • Function 
    • determining sequence for a desired DNA sample
  • Process (Sanger/direct method)
    • DNA sample put in 4 different reaction mixtures
      • each contains all 4 deoxynucleotides, 1 different dideoxyneucleotide (ddNTP), and a DNA polymerase
    • dideoxynucleotides stop DNA polymerization at each base
      • generates DNA chains of various lengths that encompass the entire original sequence
    • solutions are separated by gel electrophoresis
    • sequence can be deduced by looking at each electrophoresis row and reading which ddNTP was added to that tube column
  • Clinical use
    • most commonly used when no specific set of mutations is a common cause of disease
 

Please rate topic.

Average 4.7 of 7 Ratings

VIDEOS (2)
Topic COMMENTS (6)
Private Note