Updated: 5/5/2017

DNA Sequencing

Topic
Review Topic
0
0
Videos
2
Overview
  • Function 
    • determining sequence for a desired DNA sample
  • Process (Sanger/direct method)
    • DNA sample put in 4 different reaction mixtures
      • each contains all 4 deoxynucleotides, 1 different dideoxyneucleotide (ddNTP), and a DNA polymerase
    • dideoxynucleotides stop DNA polymerization at each base
      • generates DNA chains of various lengths that encompass the entire original sequence
    • solutions are separated by gel electrophoresis
    • sequence can be deduced by looking at each electrophoresis row and reading which ddNTP was added to that tube column
  • Clinical use
    • most commonly used when no specific set of mutations is a common cause of disease
 

Please rate topic.

Average 4.7 of 7 Ratings

Thank you for rating! Please vote below and help us build the most advanced adaptive learning platform in medicine

The complexity of this topic is appropriate for?
How important is this topic for board examinations?
How important is this topic for clinical practice?
VIDEOS (2)
Topic COMMENTS (6)
Private Note