Overview Function generate NADPH required for FA synthesis, steroid synthesis, reduction of oxidizing agents (H2O2 see figure) provide ribose 5-phosphate required for nucleotide synthesis Pathway occurs in cytoplasm of all cells no ATP consumed or generated 2 phases oxidative produces NADPH glucose 6-phosphate (G6P) → 6-phosphogluconate catalyzed by glucose 6-phosphate dehydrogenase (G6PDH) rate limiting step activated by NADP+, insulin inhibited by NADPH irreversible nonoxidative exchanging intermediate substrates between glycolysis and HMP shunt catalyzed by transketolase requires thiamine reversible Clinical relevance glucose-6-phosphate dehydrogenase (G6PDH) deficiency pathophysiology ↓ NADPH production cells (specifically RBCs) lose protection against oxidizing agents cannot regenerate glutathione XR most common human enzyme deficiency ↑ prevalence among blacks ↑ malarial resistance by shortening the circulation life of RBCs Plasmodium does not have enough time for life span plasmodium does not have defense against free radicals ↑ in free radicals kills parasite presentation episodic hemolytic anemia intravascular hemolysis normocytic 2-3 days post precipitating stress foods fava beans common in Mediterranean foods presentation pallor, hemoglobinuria 24-48 post ingestion drugs sulfonamides, primaquine, antituberculosis drugs infection free radicals generated by the immune system Heinz bodies oxidized hemoglobin that precipitates within RBCs bite cells result from the phagocytic removal of Heinz bodies by macrophages back pain test active hemolysis screen Heinz body prep
QUESTIONS 1 of 4 1 2 3 4 Previous Next Lab Values Blood Hematologic Cerebrospinal Sweat, Urine, and BMI Blood, Plasma, Serum Reference Range ALT 8-20 U/L Amylase, serum 25-125 U/L AST 8-20 U/L Bilirubin, serum (adult) Total // Direct 0.1-1.0 mg/dL // 0.0-0.3 mg/dL Calcium, serum (Ca2+) 8.4-10.2 mg/dL Cholesterol, serum Rec: < 200 mg/dL Cortisol, serum 0800 h: 5-23 μg/dL //1600 h: 3-15 μg/dL 2000 h: ≤ 50% of 0800 h Creatine kinase, serum Male: 25-90 U/LFemale: 10-70 U/L Creatinine, serum 0.6-1.2 mg/dL Electrolytes, serum Sodium (Na+) 136-145 mEq/L Chloride (Cl-) 95-105 mEq/L Potassium (K+) 3.5-5.0 mEq/L Bicarbonate (HCO3-) 22-28 mEq/L Magnesium (Mg2+) 1.5-2.0 mEq/L Estriol, total, serum (in pregnancy) 24-28 wks // 32-36 wks 30-170 ng/mL // 60-280 ng/mL 28-32 wk // 36-40 wks 40-220 ng/mL // 80-350 ng/mL Ferritin, serum Male: 15-200 ng/mLFemale: 12-150 ng/mL Follicle-stimulating hormone, serum/plasma Male: 4-25 mIU/mL Female: premenopause: 4-30 mIU/mL midcycle peak: 10-90 mIU/mL postmenopause: 40-250 pH 7.35-7.45 PCO2 33-45 mmHg PO2 75-105 mmHg Glucose, serum Fasting: 70-110 mg/dL2-h postprandial:<120 mg/dL Growth hormone - arginine stimulation Fasting: <5 ng/mLProvocative stimuli: > 7ng/mL Immunoglobulins, serum IgA 76-390 mg/dL IgE 0-380 IU/mL IgG 650-1500 mg/dL IgM 40-345 mg/dL Iron 50-170 μg/dL Lactate dehydrogenase, serum 45-90 U/L Luteinizing hormone, serum/plasma Male: 6-23 mIU/mL Female: follicular phase: 5-30 mIU/mL midcycle: 75-150 mIU/mL postmenopause 30-200 mIU/mL Osmolality, serum 275-295 mOsmol/kd H2O Parathyroid hormone, serume, N-terminal 230-630 pg/mL Phosphatase (alkaline), serum (p-NPP at 30° C) 20-70 U/L Phosphorus (inorganic), serum 3.0-4.5 mg/dL Prolactin, serum (hPRL) < 20 ng/mL Proteins, serum Total (recumbent) 6.0-7.8 g/dL Albumin 3.5-5.5 g/dL Globulin 2.3-3.5 g/dL Thyroid-stimulating hormone, serum or plasma .5-5.0 μU/mL Thyroidal iodine (123I) uptake 8%-30% of administered dose/24h Thyroxine (T4), serum 5-12 μg/dL Triglycerides, serum 35-160 mg/dL Triiodothyronine (T3), serum (RIA) 115-190 ng/dL Triiodothyronine (T3) resin uptake 25%-35% Urea nitrogen, serum 7-18 mg/dL Uric acid, serum 3.0-8.2 mg/dL Hematologic Reference Range Bleeding time 2-7 minutes Erythrocyte count Male: 4.3-5.9 million/mm3Female: 3.5-5.5 million mm3 Erythrocyte sedimentation rate (Westergren) Male: 0-15 mm/hFemale: 0-20 mm/h Hematocrit Male: 41%-53%Female: 36%-46% Hemoglobin A1c ≤ 6 % Hemoglobin, blood Male: 13.5-17.5 g/dLFemale: 12.0-16.0 g/dL Hemoglobin, plasma 1-4 mg/dL Leukocyte count and differential Leukocyte count 4,500-11,000/mm3 Segmented neutrophils 54%-62% Bands 3%-5% Eosinophils 1%-3% Basophils 0%-0.75% Lymphocytes 25%-33% Monocytes 3%-7% Mean corpuscular hemoglobin 25.4-34.6 pg/cell Mean corpuscular hemoglobin concentration 31%-36% Hb/cell Mean corpuscular volume 80-100 μm3 Partial thromboplastin time (activated) 25-40 seconds Platelet count 150,000-400,000/mm3 Prothrombin time 11-15 seconds Reticulocyte count 0.5%-1.5% of red cells Thrombin time < 2 seconds deviation from control Volume Plasma Male: 25-43 mL/kgFemale: 28-45 mL/kg Red cell Male: 20-36 mL/kgFemale: 19-31 mL/kg Cerebrospinal Fluid Reference Range Cell count 0-5/mm3 Chloride 118-132 mEq/L Gamma globulin 3%-12% total proteins Glucose 40-70 mg/dL Pressure 70-180 mm H2O Proteins, total < 40 mg/dL Sweat Reference Range Chloride 0-35 mmol/L Urine Calcium 100-300 mg/24 h Chloride Varies with intake Creatinine clearance Male: 97-137 mL/minFemale: 88-128 mL/min Estriol, total (in pregnancy) 30 wks 6-18 mg/24 h 35 wks 9-28 mg/24 h 40 wks 13-42 mg/24 h 17-Hydroxycorticosteroids Male: 3.0-10.0 mg/24 hFemale: 2.0-8.0 mg/24 h 17-Ketosteroids, total Male: 8-20 mg/24 hFemale: 6-15 mg/24 h Osmolality 50-1400 mOsmol/kg H2O Oxalate 8-40 μg/mL Potassium Varies with diet Proteins, total < 150 mg/24 h Sodium Varies with diet Uric acid Varies with diet Body Mass Index (BMI) Adult: 19-25 kg/m2 Calculator ( ) xy AC 7 8 9 ÷ 4 5 6 × 1 2 3 - 0 . = + You have 100% on this question. Just skip this one for now. Take This Question Anyway (M1.BC.4707) A 12-year-old boy presents to the emergency department with fatigue and back pain. He has also had tea-colored urine over the last 24 hours. Upon being asked about changes to his routine over the last week, he remembers running a 5K, trying alcohol with a friend, and having a large meal at a friend's house with ingredients that he did not recognize. On physical exam he appears to be pale with conjunctival pallor. Labs are as follows:Hemoglobin: 11 g/dLHematocrit: 32%Mean Corpuscular Volume: 88 fLPeripheral blood smear findings are shown in Figure A. An enzyme defect in which of the following pathways is likely responsible for this patient's symptoms? Review Topic QID: 108445 FIGURES: A Type & Select Correct Answer 1 Amino acid synthesis 3% (7/207) 2 Glycolysis 15% (32/207) 3 Hemoglobin assembly 8% (16/207) 4 Hexose mono-phosphate (HMP) shunt 58% (120/207) 5 Porphyrin synthesis 13% (26/207) M1 Select Answer to see Preferred Response SUBMIT RESPONSE 4 Sorry, this question is for PEAK Premium Subscribers only Upgrade to PEAK You have 100% on this question. Just skip this one for now. Take This Question Anyway (M1.BC.74) A 32-year-old male with HIV presents to his physician with complaints of shortness of breath and fatigue. He has a history of being non-compliant with his HAART regimen, and his CD4 count is found to be 149. The physician obtains a chest radiograph, which is shown in Figure A. Further workup demonstrates that the patient is infected with the organism demonstrated in Figure B. The physician prescribes the first-line treatment for this infection. One day later, the patient returns complaining of severe weakness and dark colored urine. On exam, the physician notes that the patient is jaundiced, and a peripheral blood smear demonstrates the findings shown in Figure C. Which of the following would most mimic this patient's condition? Review Topic QID: 106610 FIGURES: A B C Type & Select Correct Answer 1 Glutathione reductase deficiency 57% (8/14) 2 Galactokinase deficinecy 7% (1/14) 3 Ornithine transcarbamolyase deficiency 14% (2/14) 4 Carnitine deficiency 7% (1/14) 5 Cystathionine synthase deficiency 7% (1/14) M1 Select Answer to see Preferred Response SUBMIT RESPONSE 1 You have 100% on this question. Just skip this one for now. Take This Question Anyway (M1.BC.22) A 55-year-old African American lawyer presents to your office with complaints of fatigue, back pain and dark urine that he notices in the morning when he wakes up. The patient is well known to you. He frequently travels to Bangkok where he has admitted to having unprotected sex. You have treated him in the past for chlamydia, gonorrhea and syphilis. This patient has a family history of sickle cell disease in 4 of his brothers. He has a past medical history of HIV and his most recent CD4 count was 147 cells/µL. His current medications include lisinopril, glyburide, metformin, ibuprofen, and buspirone. Appropriate prophylactic measures were taken one week ago account for his low CD4 count and his traveling. The patient's CBC demonstrates:Hemoglobin: 9 g/dLHematocrit: 32%White blood cell count: 3,600 cells/µLPlatelets: 155,000/µLA peripheral blood smear is shown in Figure A. A chest radiograph is shown in Figure B.Which of the following is the best explanation for this patient's symptoms? Review Topic QID: 100087 FIGURES: A B Type & Select Correct Answer 1 Decreased levels of CD55 and CD59 14% (20/139) 2 Osteomyelitis of the vertebral column 11% (15/139) 3 Defective conversion of glucose-6-phosphate to 6-phosphogluconate 60% (84/139) 4 Side-effect of rifampin treatment 11% (15/139) 5 Splenic insufficiency 3% (4/139) M1 Select Answer to see Preferred Response SUBMIT RESPONSE 3
All Videos (1) Podcasts (0) Login to View Community Videos Login to View Community Videos HMP Shunt/Pentose Phosphate Pathway Keshav Mudgal Biochemistry - HMP Shunt 12/18/2015 72 views