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Updated: May 24 2017

Western Blot

5.0

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(1)

Images
https://upload.medbullets.com/topic/105058/images/hiv western blot.jpg
  • Introduction
    • Purpose
      • to identify proteins by
        • separating those protein molecules in a sample via electrophoresis
      • can be used as a confirmatory test for a positive HIV ELISA test
    • Example
      • HIV antigens (e.g., p24, gp41, and gp120) are separated by electrophoresis and then
        • blotted onto nitrocellulose paper
          • the patient's serum (which contains produced antibodies) is added
            • where the antibodies can bind to the antigens it recognizes
          • anti-human antibodies conjugated to an enzyme or radioactive label is then added
            • these anti-human antibodies can bind to the patient's antibody which
              • produces bands
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