Updated: 5/24/2017

Fluorescent Antibody Tests

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Introduction
  • Fluorescent antibody testing can be divided into
    • direct fluorescent antibody test (DFA)
      • use
        • for antigen localization and detection via
          • a fluorescently labeled antibody that binds to the antigen of interest
      • examples
        • used to help in diagnosing
          • varicella zoster virus (VZV) infection
          • Legionella infection
          • herpes simplex virus (HSV) infection
    • indirect fluorescent antibody test (IFA)
      • use
        • for pathogen-specific antibody detection via
          • a fluorescently labeled anti-antibody mixed with
            • the patient's serum that contains the produced antibody and
              • the laboratory-generated infected tissue sample
      • examples
        • used to detect the presence of
          • anti-nuclear antibodies
          • anti-dsDNA antibodies
          • anti-glomerular basement membrane antibodies

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