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Updated: Nov 28 2019

Reverse Transcription

  • Overview
    • Function
      • convert RNA to DNA
      • performed by reverse transcriptase
        • has high error rate and cannot proofread
        • three activities
          • RNA-dependent DNA polymerase
          • RNase
          • DNA-dependent DNA polymerase
    • Clinical relevance
      • present in retroviruses
        • e.g. HIV
          • some HIV drugs inhibit reverse transcriptase
            • AZT
              • enters cells and modified by addition of ATP
              • functions as a nucleoside analog
              • terminates replication
      • present in telomerase
        • see Telomere topic
      • used in RT-PCR
        • see PCR topic
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