Updated: 5/24/2017

Western Blot

Topic
Review Topic
0
0
Introduction
  • Purpose 
    • to identify proteins by
      • separating those protein molecules in a sample via electrophoresis
    • can be used as a confirmatory test for a positive HIV ELISA test
  • Example 
    • HIV antigens (e.g., p24, gp41, and gp120) are separated by electrophoresis and then
      • blotted onto nitrocellulose paper
        • the patient's serum (which contains produced antibodies) is added
          • where the antibodies can bind to the antigens it recognizes
        • anti-human antibodies conjugated to an enzyme or radioactive label is then added
          • these anti-human antibodies can bind to the patient's antibody which
            • produces bands

References

 

Please rate topic.

Average 5.0 of 1 Ratings

Thank you for rating! Please vote below and help us build the most advanced adaptive learning platform in medicine

The complexity of this topic is appropriate for?
How important is this topic for board examinations?
How important is this topic for clinical practice?
Topic COMMENTS (0)
Private Note