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Taq polymerase
7%
31/442
Reverse transcriptase
74%
329/442
Primers
5%
20/442
Deoxynucleotide triphosphates
10%
42/442
Target DNA strand
3%
13/442
Select Answer to see Preferred Response
Reverse transcriptase is used to convert mRNA into complementary DNA (cDNA). If you already have DNA, there is no need for reverse transcriptase to perform PCR. PCR requires DNA primers specific for the selected DNA region to be amplified, DNA sample of interest, heat stable DNA polymerase (typically Taq polymerase), and deoxyribonucleotide triphosphates. The process occurs by first denaturing the dsDNA with heat. Then the temperature is cooled to allow for the DNA primers to anneal to the region of interest. Finally, the Taq polymerase uses the primers as a starting point to replicate the region of interest. This process is then repeated 30-40 times. Incorrect Answers: Answer 1, 3-5: All of these reagents are necessary to perform a PCR.
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